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Isoflurane late preconditioning against myocardial stunning is associated with enhanced antioxidant defenses
Author(s) -
CRYSTAL G. J.,
MALIK G.,
YOON S.H.,
KIM S.J.
Publication year - 2012
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.2011.02583.x
Subject(s) - medicine , glutathione peroxidase , glutathione reductase , superoxide dismutase , isoflurane , myocardial stunning , ischemia , catalase , anesthesia , antioxidant , glutathione , coronary occlusion , pharmacology , minimum alveolar concentration , oxidative stress , biochemistry , enzyme , chemistry
Background We tested the hypothesis that an upregulation of antioxidant proteins [ Cu‐Zn superoxide dismutase ( SOD ), Mn SOD , catalase, glutathione peroxidase, and glutathione peroxidase] plays a role in the delayed protection against myocardial stunning produced by isoflurane preconditioning ( ISOPC ). Findings were compared with late ischemic PC ( IPC ). Methods Fourteen mongrel dogs were chronically instrumented to measure coronary blood flow and myocardial wall thickening ( WT ) in conscious state. In G roup 1, dogs underwent IPC , induced by a 10‐min coronary artery occlusion ( CAO ); after 24 h of reperfusion, they were subjected to a second 10‐min ischemia CAO ‐reperfusion. In G roup 2 ( ISOPC ), dogs inhaled one minimum alveolar concentration ( MAC ) ISO (1.4% in O 2 ) for 60 min, allowed to recover for 24 h, and then subjected to CAO ischemia‐reperfusion. Recovery of WT following the initial 10‐min CAO in G roup 1 served as control response for both ISOPC and IPC . Expression and activity of antioxidant proteins were measured using W estern blotting and spectrophotometric techniques, respectively. Results Two to three hours of reperfusion were required for recovery of WT following either ISOPC or IPC ; in contrast, without PC , WT remained markedly reduced (30% below baseline) at this time point and required more than 6 h of reperfusion for recovery. Neither IPC nor ISOPC affected expression of Cu‐Zn SOD , Mn SOD , or catalase. However, ISOPC increased activity of Mn SOD (+40%), catalase (+39%), glutathione peroxidase (+37%), and glutathione reductase (+93%) ( P < 0.05); IPC had similar effects. Conclusion ISOPC had powerful, delayed anti‐stunning effect that was associated with an enhancement of endogenous antioxidant defenses.