Premium
Dose‐dependent effect of S(+) ketamine on post‐ischemic endogenous neurogenesis in rats
Author(s) -
WINKELHEIDE U.,
LASARZIK I.,
KAEPPEL B.,
WINKLER J.,
WERNER C.,
KOCHS E.,
ENGELHARD K.
Publication year - 2009
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.2009.01905.x
Subject(s) - ketamine , medicine , nmda receptor , ischemia , neurogenesis , dentate gyrus , anesthesia , hippocampus , brain ischemia , glutamate receptor , pharmacology , antagonist , neuroscience , receptor , biology
Background: Ketamine is a non‐competitive antagonist at N ‐methyl‐ d ‐aspartate (NMDA) receptors and reduces neuronal injury after cerebral ischemia by blocking the excitotoxic effects of glutamate. However, cerebral regeneration by means of endogenous neurogenesis may be impaired with blockade of NMDA receptors. The effects of S(+) ketamine on post‐ischemic neurogenesis are unknown and investigated in this study. Methods: Thirty‐two male Sprague–Dawley rats were randomly assigned to the following treatment groups with intravenous S(+) ketamine anesthesia: S(+) ketamine 0.75 mg/kg/min with or without cerebral ischemia and S(+) ketamine 1.0 mg/kg/min with or without cerebral ischemia. Eight non‐anesthetized, non‐ischemic animals were investigated as naïve controls. Forebrain ischemia was induced by bilateral common carotid artery occlusion in combination with hemorrhagic hypotension. 5‐bromo‐2‐deoxyuridine (BrdU) was injected intraperitoneally for seven consecutive post‐operative days. BrdU‐positive neurons in the dentate gyrus and histopathological damage of the hippocampus were analyzed after 28 days. Results: The number of new neurons was not affected by S(+) ketamine in the absence of cerebral ischemia. The ischemia‐induced increase in neurogenesis was reduced by high‐dose S(+) ketamine. Cell death of ischemic animals did not vary between low‐ and high‐dose S(+) ketamine. Conclusion: While low concentrations of S(+) ketamine allow an ischemia‐induced increase in the number of new neurons, high S(+) ketamine concentrations block the post‐ischemic increase in newly generated neurons. This effect is irrespective of the extent of other histopathological damage and in line with studies showing that NMDA receptor antagonists like MK‐801 inhibit neurogenesis after cerebral ischemia.