Premium
Mivazerol inhibits intrathecal release of glutamate evoked by halothane withdrawal in rats
Author(s) -
Zhang X.,
Kindel G.,
Wülfert E.,
Hanin I.
Publication year - 1998
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1998.tb05362.x
Subject(s) - halothane , microdialysis , medicine , anesthesia , glutamate receptor , agonist , perfusion , heart rate , pharmacology , endocrinology , blood pressure , receptor , central nervous system
Background : Mivazerol is a new and selective α 2 ‐adrenergic receptor agonist devoid of hypotensive effects (1, 2). Previous studies have demonstrated that mivazerol prevents hemodynamic instability during emergence from halothane anesthesia in rats (3). The present study was to determine whether glutamate and aspartate are involved in this action of mivazerol, at the second to third thoracic segments (T2–T3) of the spinal cord. Methods : In vivo microdialysis in combination with high‐performance liquid chromatography (HPLC) was employed in the study. Blood pressure (BP) and heart rate (HR) were recorded along with intrathecal (i.t.) microdialysis perfusion. Results : BP, HR and i.t. release of glutamate (GLU, pmol/μl) were stable in the rats under 1.1% halothane anesthesia. However, halothane withdrawal immediately increased BP, HR, and i.t. release of GLU, and remained elevated for at least 2 h after withdrawal of halothane. Thirty minutes prior to halothane withdrawal, intravenous (i.v.) infusion of mivazerol (15 μg · kg –l · h – ) almost completely prevented the increases in HR (Δ18±7 vs Δ79±7 beats/min), and in the i.t. release of GLU (Δ10.3±3.7 vs Δ30.6±5.9; 112% vs 167%). Local i.t. microinjection of mivazerol (2.5 μg/kg) 2 min prior to withdrawal of halothane also blocked the HR responses, as well as on the i.t. release of GLU following halothane withdrawal. Conclusion : The present study demonstrates that emergence from halothane anesthesia increases i.t. release of GLU, and that mivazerol has an inhibitory effect on the above, through its direct action on the spinal cord.