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Effects on complement activation and cytokine (TNF‐α and IL‐8) release of infusion of anti‐TNF‐antibodies or a xanthine derivative (HWA 138) in septic baboons
Author(s) -
Bengtsson A.,
Redl H.,
Schlag G.,
Mollnes T. E.,
Högåsen K.
Publication year - 1996
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1996.tb04427.x
Subject(s) - medicine , tumor necrosis factor alpha , septic shock , cytokine , complement system , antibody , sepsis , body weight , pharmacology , immunology , endocrinology
Background. Sepsis and septic shock lead to activation of the complement cascade and to the release of pro‐inflammatory cytokines. Methods. The effects of E coli infusion and of infusion of anti‐TNF antibodies and a xanthine derivative (HWA 138) on complement activation and cytokine release was evaluated in 17 baboons. All animals received 5×10 8 live bacteria per kg body weight. Five animals received only bacteria, five received in addition 0.5 mg per kg body weight of anti‐TNF‐antibody, and seven received an infusion of 6 mg per kg body weight of HWA 138 in addition to the bacteria. Results. In baboons receiving 5×10 8 live E coli per kg body weight increased plasma levels of TCC, TNF‐α and IL‐8 were found. The release of TNF‐α was lower in the group receiving HWA 138 at 2 h after the infusion. In baboons receiving an infusion of anti‐TNF antibody the concentration of IL‐8 was lower at 2 and 4 h than in animals receiving just E coli or HWA 138. Conclusion. Infusion of anti‐TNF‐antibody before E coli infusion will decrease the formation of IL‐8. Infusion of HWA 138 before the E coli infusion will also inhibit the formation of TNF‐α.