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Hyperglycemia in global cerebral ischemia and reperfusion: a 31‐phosphorous NMR spectroscopy study in rats
Author(s) -
Haraldseth O.,
Nygård Ø.,
Grønås T.,
Southon T.,
Gisvold S. E.,
Unsgård G.
Publication year - 1992
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1992.tb03417.x
Subject(s) - phosphocreatine , medicine , ischemia , intracellular ph , acidosis , intracellular , intravenous bolus , bolus (digestion) , endocrinology , anesthesia , energy metabolism , biochemistry , chemistry
31‐phosphorous magnetic resonance spectroscopy was used in a rat model of 10 min severe incomplete forebrain ischemia (two‐vessel occlusion with hypotension) to assess the effect of hyperglycemia on intracellular pH and high energy phosphates during ischemia and early reperfusion. One group (n = 8) with preischemic hyperglycemia (serum glucose 20 mmol ± l ‐1 ) showed an increased intracellular acidosis (pH 6.35) during ischemia compared to 6.55 in the normoglycemic control group (n=7, P <0.001), but the recovery of phosphocreatine and ATP in early reperfusion was the same in the two groups. Another group (n = 7) was normoglycemic during ischemia, but received an i.v. bolus of glucose during the first minute of reperfusion. In this group the recovery of intracellular pH in early reperfusion was slower than in the control group (0.034 ± 0.006 pH units per minute compared to 0.052 ± 0.11 in the controls, ± s.d. and P <0.01).