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The caffeine contracture test for malignant hyperthermia: caffeine citrate, caffeine benzoate or caffeine free base?
Author(s) -
Heytens L.,
Heffron J. J. A.,
Camu F.
Publication year - 1991
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1991.tb03344.x
Subject(s) - caffeine , medicine , malignant hyperthermia , contracture , anesthesia , pharmacology , surgery
The aim of the present study was to investigate whether the three different caffeine preparations — caffeine citrate, caffeine benzoate and the free base — used for in vitro diagnosis of malignant hyperthermia susceptibility — produced the same amount of contracture in rat diaphragm. At equimolar caffeine concentrations, the pure base generated more tension in the rat diaphragm muscle than caffeine benzoate or caffeine citrate. The citrate lowers the pH and the free Ca 2+ concentration of the test bath and thus suppresses the caffeine contracture. The benzoate is believed to inhibit the caffeine contracture by its carbonyl group in a way similar to the effect of benzocaine.