Premium
Effects of halothane on the efflux of [ 3 H]D asprtate from rat brain slices
Author(s) -
Arai T.,
Hatano Y.,
Mori K.
Publication year - 1990
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1990.tb03083.x
Subject(s) - medicine , halothane , efflux , anesthesia , pharmacology , biochemistry , chemistry
The in vitro effects of halothane on the potassium‐stimulation‐induced efflux of [ 3 H]D‐aspartate in rat cerebral cortex slices were studied. The slices were initially incubated with Krebs‐Ringer's solution containing [ 3 H]D‐aspartate, a putative excitatory transmitter. The slices were then stimulated with high concentrations of K + in the presence and absence of halothane, and the efflux was measured using a scintillation counter. Halothane, 1% and 2%, had little effect on the potassium‐stimulation‐induced efflux, but that of 4 and 8% increased the efflux significantly. The spontaneous efflux was unaffected by all concentrations of halothane studied. The control study of pentobarbital, in the concentration of 0.05 to 1.00 mmol/1, reduced the efflux in a dose‐related manner. These findings indicate that the release of an excitatory transmitter, aspartate, may not be involved in the mechanism of halothane anaesthesia.