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Effects of thiopental on granulocyte oxidative microbicidal capacity
Author(s) -
Salo M.,
Perttilä J.
Publication year - 1989
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1989.tb02920.x
Subject(s) - phagocytosis , medicine , oxidative phosphorylation , staphylococcus aureus , zymosan , chemiluminescence , granulocyte , respiratory burst , microbiology and biotechnology , anesthesia , in vitro , bacteria , immunology , biochemistry , chromatography , biology , chemistry , genetics
Some anaesthetics are known to depress granulocyte chemiluminescence responses in the phagocytosis of zymosan as a measure of their oxidative microbicidal capacity. In this study the effects of thiopental were measured on chemiluminescence responses in the phagocytosis of Staphylococcus aureus and Escherichia coli , which are the commonest bacteria causing postoperative infections. Granulocytes from 14 healthy volunteers (mean age 35 ± 9 (s.d.) years) were tested in vitro in the presence of clinical thiopental concentrations 0, 5, 10 and 25 μg/ml (0, 18, 36 and 91 μmol/l, respectively). During a 60‐min exposure period, decreased chemiluminescence responses occurred to Staph. Aureus at 5 μg/ml ( P < 0.01) and higher thiopental concentrations and to E. coil at 10 μg/ml ( P <0.01) and higher concentrations, as a sign of depressed oxidative microbicidal activity.