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Inhibition of Human Leukocyte Metabolism and Random Mobility by Local Anaesthesia
Author(s) -
HAMMER R.,
DAHLGREN C.,
STENDAHL O.
Publication year - 1985
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1111/j.1399-6576.1985.tb02246.x
Subject(s) - medicine , phagocytosis , peroxidase , myeloperoxidase , metabolism , anesthesia , dose dependence , pharmacology , biochemistry , enzyme , immunology , inflammation , chemistry
Hexose‐monophosphate shunt (HMS) activity, myelo‐peroxidase‐(MPO)‐mediated iodination and random mobility in human polymorphonuclear leukocytes (PMNs) were studied in the presence of lignocaine. Incubating the PMNs with 0.1% lignocaine during phagocytosis inhibited the 14 CO 2 produced from glucose‐1‐ 14 ‐C via the HMS shunt by 33%. On increasing the concentration of lignocaine, a dose‐dependent inhibition was noted. The MPO‐mediated iodination was inhibited by 73% in the presence of 0.1% lignocaine, and complete inhibition took place when the concentration was increased to 0.5%. The random mobility of leukocytes was studied by an opto‐electronic technique. In the presence of 0.5% lignocaine, all leukocytes examined were completely immobilized;in the presence of 0.1% lignocaine immobilization took place within 45–65 min.