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Production of homozygous α‐1,3‐galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer
Author(s) -
Nottle Mark B.,
Beebe Luke F. S.,
Harrison Sharon J.,
McIlfatrick Stephen M.,
Ashman Rodney J.,
O’Connell Phillip J.,
Salvaris Evelyn J.,
Fisicaro Nella,
Pommey Sandra,
Cowan Peter J.,
D’Apice Anthony J. F.
Publication year - 2007
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2007.00417.x
Subject(s) - somatic cell nuclear transfer , biology , litter , andrology , somatic cell , fetus , boar , embryo , genetics , zoology , microbiology and biotechnology , embryogenesis , pregnancy , gene , blastocyst , semen , medicine , agronomy
We report here our experience regarding the production of double or homozygous Gal knockout (Gal KO) pigs by breeding and somatic cell nuclear transfer (SCNT). Large White × Landrace female heterozygous Gal KO founders produced using SCNT were mated with Hampshire or Duroc males to produce a F1 generation. F1 heterozygous pigs were then bred to half‐sibs to produce a F2 generation which contained Gal KO pigs. To determine the viability of mating Gal KO pigs with each other, one female F2 Gal KO pig was bred to a half‐sib and subsequently a full‐sib Gal KO. F1 and F2 heterozygous females were also mated to F2 Gal KO males. All three types of matings produced Gal KO pigs. To produce Gal KO pigs by SCNT, heterozygous F1s were bred together and F2 fetuses were harvested to establish primary cultures of Gal KO fetal fibroblasts. Gal KO embryos were transferred to five recipients, one of which became pregnant and had a litter of four piglets. Together our results demonstrate that Gal KO pigs can be produced by breeding with each other and by SCNT using Gal KO fetal fibroblasts.