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Delayed functional maturation of neonatal porcine islets in recipients under strict glycemic control
Author(s) -
Kin Tatsuya,
Korbutt Gregory S.
Publication year - 2007
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2007.00414.x
Subject(s) - islet , renal capsule , medicine , transplantation , glycemic , endocrinology , xenotransplantation , diabetes mellitus , capsule , insulin , biology , botany
Background: The aim of this study was to compare the functional maturation of neonatal porcine islet (NPI) grafts exposed to long‐term hyperglycemia with those implanted under euglycemic conditions.Methods: mice Neonatal porcine islets were transplanted under the left renal capsule of diabetic SCID mice (group H), or in diabetic SCID mice who were also implanted with 500 BALB/c islets under the right renal capsule (group N). On day 42, the right kidneys were removed in both groups. Results: No animals in group H achieved euglycemia within 3 weeks after transplantation. Thus, these mice were exposed to long‐term hyperglycemia. Mice in group N became euglycemic immediately after transplantation, however after removal of BALB/c grafts on day 42 they exhibited significantly higher blood glucose levels than in group H and showed glucose intolerance after glucose administration. Cellular insulin content of NPI grafts harvested on day 58 or 72 was significantly lower in group N mice compared to group H. Conclusions: These results suggest that tight control of glycemia reduces the functional maturation of NPI grafts.