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Transplantation of human CD34 + stem cells from umbilical cord blood to rats with thioacetamide‐induced liver cirrhosis
Author(s) -
SáezLara María José,
Frecha Cecilia,
Martín Francisco,
Abadía Francisco,
Toscano Miguel,
Gil Ángel,
Fontana Luis
Publication year - 2006
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2006.00344.x
Subject(s) - transplantation , stem cell , cirrhosis , cd34 , haematopoiesis , progenitor cell , biology , thioacetamide , umbilical cord , genetic enhancement , pathology , microbiology and biotechnology , cancer research , medicine , immunology , endocrinology , gene , biochemistry
Background: Liver fibrosis results from accumulation of extracellular matrix components and is associated with many chronic hepatic diseases. There is to date no specific therapy for this disease, and patients receive treatment for its associated complications. Specific progenitor cells, known as oval cells, are present in the liver. As oval cells express markers such as CD34, they are thought to arise from a hematopoietic precursor. The aim of this work was to investigate whether transplantation of hematopoietic CD34 + stem cells could improve hepatic fibrosis by their differentiation into hepatocytes. Methods: CD34 + stem cells from human umbilical cord blood were purified, transduced with a lentiviral vector containing the green fluorescent protein (GFP) gene and injected via portal vein into rats with liver cirrhosis induced by the 4‐month administration of thioacetamide. Rats were killed 15 and 60 days post‐transplantation. Results: Up to 37% and 22% fluorescent cells were observed in the blood of control and cirrhotic rats, respectively, at 15 days post‐transplantation. At 60 days post‐transplantation, however, fluorescent cells were completely absent from the blood. Fluorescence was not detected in liver sections at either 15 or 60 days post‐transplantation. Polymerase chain‐reaction study to detect the GFP gene ruled out silencing of the transgene. Conclusions: These results suggest that the transplanted cells did not engraft in the liver and were eliminated from the rats.