Premium
Long‐term cultured neonatal porcine islet cell monolayers: a potential tissue source for transplant in diabetes
Author(s) -
Mancuso Francesca,
Basta Giuseppe,
Calvitti Mario,
Luca Giovanni,
Guido Lucia,
Racanicchi Leda,
Montanucci Pia,
Becchetti Ennio,
Calafiore Riccardo
Publication year - 2006
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2006.00305.x
Subject(s) - islet , insulin , diabetes mellitus , cell , transplantation , glucagon , phenotype , biology , medicine , endocrinology , islet cell transplantation , cell culture , pancreatic islets , type 1 diabetes , biochemistry , gene , genetics
Background: The restricted availability of cadaveric human donor pancreases mandates validation of possibly inexhaustible, alternative sources of insulin secretory cells in order to expand islet transplant for the therapy of insulin dependent diabetes mellitus (T1DM). Methods: Neonatal pig pancreatic islets (NPI), isolated and purified by our method, were specially cultured until confluent cell monolayers were obtained. Expression of several β ‐cell phenotype transcriptional factors, under glucose and other stimuli, were examined throughout 90 days of culture. Results: High glucose concentration and glucagon‐like peptide 1 (GLP‐1) were associated with maintenance either of insulin secretory patterns from the incubated cell monolayers, or expression of transcriptional markers associated with β ‐cell like phenotypes. Conclusion: Morphological and molecular expression of β ‐cell markers and products from NPI cell monolayers seem to identify a novel and potentially powerful source of insulin producing cells that might fulfill transplant needs for insulin substitution therapy