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Expression of human soluble complement receptor 1 by a pig endothelial cell line inhibits lysis by human serum
Author(s) -
Manzi Lorna,
Montaño Ramón,
Abad María Jesús,
Arsenak Miriam,
Romano Egidio,
Taylor Peter
Publication year - 2006
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2005.00265.x
Subject(s) - xenotransplantation , complement system , cd59 , cd46 , biology , transfection , receptor , microbiology and biotechnology , decay accelerating factor , c5a receptor , transgene , complement receptor 1 , antibody , complement (music) , complement receptor , cell culture , complement factor b , complement factor i , classical complement pathway , transplantation , gene , immunology , phenotype , biochemistry , medicine , genetics , complementation
The importance of complement activation and naturally occurring anti‐pig antibodies in the hyperacute rejection (HAR) observed in models of pig‐to‐human xenotransplantation is well established. To overcome this, much effort has been dedicated to preparing transgenic pigs by knocking out Galα(1‐3)Gal expression in these animals, or knocking in the expression of human complement regulatory proteins (CRPs), such as CD59 or decay accelerating factor. A soluble form of another membrane CRP, complement receptor type 1 (CR1), has also been shown to inhibit complement activation. Here, we show that transfection of a pig endothelial cell line with a truncated form of human soluble complement receptor 1 (sCR1) almost completely protected these cells from complement‐mediated lysis by human AB serum. Pigs genetically manipulated to express human sCR1 may represent an additional strategy to inhibit HAR of pig‐to‐human transplanted organs.