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Porcine complement regulators protect aortic smooth muscle cells poorly against human complement‐induced lysis and proliferation: consequences for xenotransplantation
Author(s) -
Capey Steven,
Van Den Berg Carmen W.
Publication year - 2005
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2005.00217.x
Subject(s) - xenotransplantation , cd59 , microbiology and biotechnology , transplantation , complement system , flow cytometry , biology , cell growth , cell , complement membrane attack complex , cd46 , immunology , immune system , medicine , biochemistry
Background: Accelerated atherosclerosis after transplantation has been observed and is characterized by smooth muscle cell proliferation in the graft. Porcine cells are frequently used in models of atherosclerosis and porcine organs are considered for use in transplantation. Complement (C) activation is known to play a major role in rejection of xenografts and is also considered to play a role in the development of atherosclerosis. The aim of this study was to investigate the expression and function of membrane bound regulators of complement (CReg) on porcine aortic smooth muscle cells (PASMC). Methods: The PASMC were assessed for expression of CReg and susceptibility to lysis by human C by flow‐cytometry. The effect of various cytokines on CReg expression and C‐susceptibility was investigated. The ability of human C to induce cell proliferation was assessed using the Alamar blue assay. Results: The PASMC only express the CReg membrane cofactor protein (MCP) and CD59 on their cell surface. MCP expression was increased by interleukin (IL)‐4. In contrast to porcine aortic endothelial cells (PAEC), PASMC were found to be surprisingly sensitive to C‐mediated lysis, mainly due to a low level of expression of CD59. Human C‐induced proliferation of PASMC, which was dependent on complete membrane attack complex (MAC) formation. Conclusions: Endogenously expressed CReg on PASMC poorly protect these cells to human C. Human C can induce proliferation of PASMC. In order to prevent accelerated atherosclerosis in porcine xenografts, increased levels of CReg not only have to be obtained on the endothelial cells but also on the smooth muscle cells.