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T‐cell differentiation of human and
non‐human primate CD34 + hematopoietic progenitor cells using porcine thymic stroma
Author(s) -
Rosenzweig Michael,
Yamada Kazuhiko,
Harper David M.,
Hempel Donna M.,
Johnson R. Paul
Publication year - 2001
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.2001.00106.x
Subject(s) - biology , cd34 , progenitor cell , haematopoiesis , lymphopoiesis , immunology , cd8 , immune system , transplantation , t cell , stem cell , microbiology and biotechnology , cancer research , medicine
Transplantation of swine thymic tissue has been proposed as an approach to reconstitute the immune system of HIV‐infected individuals. This is an attractive strategy because miniature swine are readily available as donors and porcine tissue is resistant to infection with HIV‐1. Demonstration that porcine thymus tissue supports primate T‐cell differentiation is critical to the ultimate utility of this approach. Using a thymic stroma culture system we have previously described [Rosenzweig M, Marks DF, Zhu H et al. In vitro T lymphopoiesis of human and rhesus CD34+ progenitor cells. Blood 1996; 87: 4040], we demonstrate that porcine thymus tissue is able to promote the in vitro T‐lymphocyte differentiation of both human and non‐human primate hematopoietic progenitor cells. CD34 + hematopoietic progenitors differentiated into both double positive (CD4 + CD8 + ) and single positive thymocytes expressing CD4 or CD8 alone. A polyclonal T‐cell repertoire was evident. In addition, the T cells responded appropriately to mitogen and were permissive to infection with simian immunodeficiency virus (SIV). These data demonstrate the ability of porcine thymus to support T‐cell differentiation of both human and non‐human hematopoietic progenitor cells and support in vivo studies of transplantation of swine thymic tissue as a strategy for immune reconstitution in AIDS.