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Species‐specific detection of porcine xenografts with an antibody against a novel epitope of the lymphocyte homing receptor CD44
Author(s) -
Oettinger Henry F.,
Sullivan Jennifer A.,
Crosby Katherine E.,
Kelley Julie A.,
Jaooby Douglas B.,
Dinsmore Jonathan,
Zawadzka Agatha,
Edge Albert S.B.
Publication year - 1997
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1997.tb00190.x
Subject(s) - cd44 , epitope , microbiology and biotechnology , monoclonal antibody , biology , antibody , lymphocyte homing receptor , xenotransplantation , immunohistochemistry , neural cell adhesion molecule , cell adhesion molecule , lymphocyte , chemistry , transplantation , immunology , cell , cell adhesion , biochemistry , medicine , surgery
We have isolated and characterized a murine monoclonal antibody, 10–14, that is reactive with an 85 kDa glycoprotein found on porcine peripheral blood lymphocytes, cultured aortic endothelial cells, fetal cardiomyocytes, and mixed neural/glial cell cultures. In contrast to the anti‐CD44 antibody Z062, which reacted with both porcine and murine CD44, the 10–14 antibody did not react with monkey, rabbit, rat, or murine cells, suggesting that 10–14 recognized a novel epitope found exclusively on the porcine CD44 molecule; 10–14 is thus a novel reagent for identification of xenotransplanted porcine cells. The reagent has been used in this study as an immunohistochemical probe to detect porcine neural cell grafts in the central nervous system of the rat and to label porcine fetal cardiomyocytes. Moreover, the antibody provides information on the expression of the CD44 molecule that is critical for lymphocyte adhesion and trafficking.