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Conditions permitting short‐term engraftment of human T cells in RAG‐1 mutant mice
Author(s) -
Sawada Tokihiko,
Friedman Tony,
Iacomini John
Publication year - 1997
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1997.tb00189.x
Subject(s) - spleen , lymph , cell , mutant , biology , microbiology and biotechnology , xenotransplantation , immunology , transplantation , chemistry , medicine , pathology , biochemistry , gene
The ability to reconstitute RAG‐1 deficient (R ‐ ) mice with human peripheral blood cells was examined using four different host preparative regimens. Our results indicate that while untreated R ‐ mice could be reconstituted with human cells, pre‐conditioning with either 5Gy whole body irradiation (WBI) or anti‐asialo GM1 rabbit anti‐serum led to an increase in the frequency of CD45 + human cells that could be detected in the circulation. Pre‐conditioning with both anti‐asialo GM1 and WBI led to a further increase in the frequency of circulating human cells in reconstituted R ‐ mice. Human CD45 + cells were detected in the spleen and lymph nodes, but not the thymus of reconstituted mice pre‐conditioned with WBI and anti‐asialo GM1. Our results suggest that mouse NK cells and a limitation in physical space are limiting factors mediating resistance I to human cell engraftment in R ‐ mice.