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Cellular xenoresponses: Observation of significant primary indirect human T cell anti‐pig xenoresponses using co‐stimulator‐deficient or SLA class II‐negative porcine stimulators
Author(s) -
Dorling Anthony,
Binns Richard,
Lechier Robert I.
Publication year - 1996
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1996.tb00127.x
Subject(s) - priming (agriculture) , human leukocyte antigen , in vitro , immunology , peripheral blood , antigen , t cell , biology , limiting , cell , microbiology and biotechnology , immune system , biochemistry , mechanical engineering , botany , germination , engineering
We have documented the strength and characteristics of the in vitro human anti‐porcine indirect T cell response using porcine stimulators that were unable to provoke a direct xenoresponse. Our results indicate that HLA‐DR‐restricted and HLA‐DQ‐restricted CD4 + T cells recognise porcine xenoantigens presented by conventional APC, rather than xenospecific B cells. Limiting dilution analysis assays indicate a high precursor frequency of T cells with indirect xenospecificity in the peripheral blood of human responders. These data demonstrate that the indirect response requires no priming, but instead reflects the large number of pig‐specific antigens.