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Evidence that double transfection to xenoendothelial cells using GPI‐anchoring complement regulatory factor (DAF and HRF20) genes is useful for the inhibition of human complement‐mediated cytolysis
Author(s) -
Hayashi Shuji,
Emi Nobuhiko,
Isobe KenIchi,
Okada Hidechika,
Yokoyama Itsuo,
Takagi Hiroshi
Publication year - 1995
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1995.tb00110.x
Subject(s) - decay accelerating factor , transfection , complementary dna , cd59 , microbiology and biotechnology , biology , complement system , cd46 , complement component 2 , cytolysis , lysis , cell culture , alternative complement pathway , antibody , gene , immunology , cytotoxicity , biochemistry , in vitro , genetics
It has been known that xenogeneic cells transfected with regulator of complement activation (RCA) molecules such as DAF ( CD55 ), MCP ( CD46 ) and HRF20 ( CD59 ) resist the lysis by the human complement. However, it has not been demonstrated that the induction of double RCA molecules is more effective than that of single RCA molecule to inhibit the human complement attack. In this study, the authors compared the effect on the protection from complement‐mediated lysis between single transfection and double transfection of DAF and HRF20 cDNA to bovine aortic endothelial cells (BAEC) using retro viral vector. BAEC transfected with DAF or HRF20 cDNA resisted the lysis by human serum in parallel with the intensity of antigen expression, whereas BAEC transfected with both DAF and HRF20 cDNA resisted the lysis by human serum with anti‐BAEC antibody, not human serum alone, more effectively than those with DAF cDNA alone. We conclude that the xenogeneic cells doubly transfected with both DAF and HRF20 cDNA are protected from complement mediated lysis more effectively than those singly transfected with DAF cDNA alone.