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Islet Isolation from the porcine pancreas, using a continuous digestion method
Author(s) -
Polo F.,
Monroy B.,
Maria S. Di,
Ruiu G.,
Darquy S.,
Petruzzo P.,
ArbetEngels C.,
Cappai A.,
Brotzu G.,
Reach G.
Publication year - 1995
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1995.tb00100.x
Subject(s) - islet , digestion (alchemy) , pancreas , chromatography , computer science , pancreatic duct , chemistry , biology , biochemistry , endocrinology , diabetes mellitus
This paper describes a method for pig islet isolation with the following original features: first, we set up a reproducible, rapid, sterile, and simple method for pancreas procurement and preparation with double duct incannulation. Second, we demonstrated that UW solution can be used for pancreas digestion; third, we designed an original method for pancreas digestion, derived from the method originally described by Ricordi, except that a double digestion circuit made it possible to digest continuously, within one step, the pancreas, which makes the method operator‐independent; finally we designed a simple method for islet purification based on a two‐layer Histopaque/ UW density gradient and the use of the COBE 2991 cell processor.