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Identification of swine and primate cellular adhesion molecules (CAM) using mouse anti‐human monoclonal antibodies
Author(s) -
KumagaiBraesch Makiko,
Schacter Bernioe,
Yan Zengmin,
Michaelson James,
Arn Scott,
Smith Mary,
WhiteScharf Mary,
Monroy Rodney,
Sachs David H.,
Kurnick James T.
Publication year - 1995
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1995.tb00071.x
Subject(s) - monoclonal antibody , cd49b , integrin , microbiology and biotechnology , cd18 , cell adhesion molecule , epitope , biology , antibody , antigen , immunology , biochemistry , receptor , interleukin 21 , cd8
Abstract: A series of adhesion molecules of swine and Cynomolgus monkeys were identified by screening for cross‐reactivity with a panel of monoclonal murine anti‐human adhesion molecule antibodies obtained from the 5th International Workshop and Conference on Human Leukocyte Differentiation Antigens (Boston, MA, USA, 1993). Of 162 antibodies tested, 25 were found that cross‐react significantly with swine cells, while 67 were found to react strongly with cells of Cynomolgus monkeys. Cross reactivities to swine were found with antibodies to CD 18 (β2 integrin), CD29 (β1 integrin), β7 integrin, CD49d (α4 integrin) CD49b(α2 integrin), and to a lesser extent to CD62p(P‐selectin), CD62L(L‐selectin), CD102(ICAM‐2), CD11b, and CD49c (α3 integrin). Cross‐reactivities to primate cells also included CD18, CD29, CD49d, and CD49b. In addition, reactivities to Cynomolgus monkey cells were detected with antibodies to CD11 (a, b, and c), CD31, CD44, CD49e, CD49f, CD50, CD54, CD56, CD62p, CD 102 and CD56. The tissue distribution and molecular weight of the swine antigens are similar to their human counterparts. These findings provide a spectrum of monoclonal antibodies that react with shared epitopes on homologous adhesion molecules of human, swine, and monkey cells, thus facilitating study of the role of these molecules in the immunobiology of monkeys and swine. These reagents may be useful to dissect the role of adhesion molecules in both alio‐ and xenoreactivity.