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Prophylactic trypsin inhibition during the isolation procedure guarantees reproducible, high porcine islet yields
Author(s) -
Heiser Axel,
Ulrichs Karin,
MüllerRuchholtz Wolfgang
Publication year - 1994
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/j.1399-3089.1994.tb00051.x
Subject(s) - islet , trypsin , collagenase , pancreas , yield (engineering) , chemistry , digestion (alchemy) , biochemistry , biology , enzyme , endocrinology , chromatography , diabetes mellitus , materials science , metallurgy
For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields.