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S‐deficiency responsive accumulation of amino acids is mainly due to hydrolysis of the previously synthesized proteins – not to de novo synthesis in Brassica napus
Author(s) -
Lee BokRye,
Muneer Sowbiya,
Kim KilYong,
Avice JeanChristophe,
Ourry Alain,
Kim TaeHwan
Publication year - 2013
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2012.01669.x
Subject(s) - amino acid , brassica , protein biosynthesis , biochemistry , metabolism , hydrolysis , chemistry , de novo synthesis , chlorophyll , biosynthesis , biology , enzyme , botany , organic chemistry
To characterize the mechanisms of amino acid accumulation under sulphur (S)‐deficiency and its physiological significance in Brassica napus , stable isotopes 15 N and 34 S were employed. The plants were exposed for 9 days to S‐deficient conditions (0.05 m M vs 1.5 m M sulphate). After 9 days of S‐deficiency, leaf‐osmotic potential and total chlorophyll content significantly decreased. S uptake decreased by 94%, whereas N uptake and biomass were not significantly changed. Using 15 N and 34 S labelling, de novo synthesis of amino acids and proteins derived from newly absorbed NO 3 − and SO 4 2 − and the content of N and S in the previously synthesized amino acids and proteins were quantified. At the whole plant level, S‐deficiency increased the pool of amino acids but resulted in strong decrease of incorporation of newly absorbed NO 3 − and SO 4 2 − into amino acids by 22.2 and 76.6%, respectively, compared to the controls. Total amount of N and S incorporated into proteins also decreased by 28.8 and 62.1%, respectively. The levels of 14 N ‐ and 32 S ‐proteins (previously synthesized proteins) strongly decreased, mainly in mature leaves. The data thus indicate that amino acid accumulation under short‐term S‐deficiency results from the degradation of previously synthesized proteins rather than from de novo synthesis.