Premium
Natural substrates of plant proteases: how can protease degradomics extend our knowledge?
Author(s) -
Tsiatsiani Liana,
Gevaert Kris,
Van Breusegem Frank
Publication year - 2012
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2011.01534.x
Subject(s) - proteases , protease , biology , arabidopsis thaliana , proteolysis , proteome , computational biology , proteomics , genome , abiotic stress , arabidopsis , gene , biochemistry , enzyme , mutant
Despite the key role of proteolysis in various intensively studied biological processes, such as plant immunity, seed development and abiotic stress responses, our knowledge on the identity of natural protease substrates in plants remains scarce. In the genome of the model plant Arabidopsis thaliana , for instance, approximately 700 genes code for proteases. However, only a few natural substrates have been identified, mainly because of the previous lack of sensitive proteomics technologies enabling the identification of low abundant proteins, together with a delay in the implementation of these technologies in the field of plant research. Here, we review the current knowledge on the identity of natural plant protease substrates and describe recently established degradomics technologies that should allow proteome‐wide studies of plant proteases in the near future.