Premium
Expression and phylogenetic analysis of two new lycopene β ‐cyclases from Citrus paradisi
Author(s) -
Mendes Amanda F. S.,
Chen Chunxian,
Gmitter Fred G.,
Moore Gloria A.,
Costa Marcio G. C.
Publication year - 2011
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2010.01415.x
Subject(s) - citrus paradisi , biology , gene , chromoplast , gene duplication , botany , ripening , citrus × sinensis , genetics , chloroplast , orange (colour) , rutaceae , horticulture , plastid
Two new lycopene β ‐cyclases ( LCYB s) were cloned and characterized from grapefruit ( Citrus paradisi Macf.). During fruit ripening, CpLCYB1 expression did not show significant differences between ‘Flame’ (red flesh) and ‘Marsh’ (white flesh), and was much lower than CpLCYB2 and nearly constant; however, CpLCYB2 expression dramatically changed in a similar tendency in the pulp of both grapefruit cultivars, but the relative abundance of mRNA in ‘Flame’ was significantly lower than in ‘Marsh’. Phylogenetically and structurally, CpLCYB1 was a chloroplast‐specific member and CpLCYB2 a chromoplast‐specific member, the two subfamilies of all the LCYB genes. An intron was found in the 5′‐untranslated region of CpLCYB1 and in two other Citrus LCYB1 genes ( CcLCYB1 and CsLCYB1‐2 ), resulting in an extra 20 amino acids, compared with all the other LCYB1s. It suggested that a different genomic event, in addition to gene duplication, has contributed to the evolution of these LCYB genes, and likewise, the change of their functions.