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Differential expression of saporin genes upon wounding, ABA treatment and leaf development
Author(s) -
Tartarini Andrea,
Pittaluga Eugenia,
Marcozzi Giordana,
Testone Giulio,
RodriguesPousada Renato Alberto,
Giannino Donato,
Spanò Laura
Publication year - 2010
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2010.01388.x
Subject(s) - saporin , biology , gene isoform , gene , differential display , complementary dna , ribosome inactivating protein , gene expression , microbiology and biotechnology , biochemistry , ribosome , rna , immunotoxin , cytotoxicity , in vitro
Saporins are type 1 ribosome‐inactivating proteins (RIPs: EC 3.2.2.22) produced in various organs of Saponaria officinalis L. Two distinct saporin types, saporin‐L and saporin‐S isoforms, were respectively purified from the intra‐ and extra‐cellular fractions of soapwort leaves. The saporin‐L isoform was lowly identical, differed for toxicity, molecular mass and amino acid composition from saporin‐S proteins forming a new monophyletic group. Genes encoding both L‐ and S‐type isoforms were cloned from leaf‐specific cDNA library; the encoded products included the N‐terminal diversity observed by protein sequencing and showed compatible weights with those from mass spectra. These genes were intron‐less belonging to small gene families. Reverse transcription polymerase chain reaction/quantitative reverse transcription polymerase chain reaction experiments evidenced their differential expression during leaf development, wounding and abscisic acid treatment. These results suggest that the saporin‐L and ‐S proteins may play diversified roles during stress responses.