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WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana
Author(s) -
Kasajima Ichiro,
Ide Yoko,
Yokota Hirai Masami,
Fujiwara Toru
Publication year - 2010
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2010.01349.x
Subject(s) - mutant , transcriptome , biology , gene , transcription factor , arabidopsis thaliana , transcription (linguistics) , promoter , genetics , wild type , arabidopsis , gene expression , microbiology and biotechnology , linguistics , philosophy
Boron (B) is one of the essential nutrients for plant growth and reproduction. Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency‐inducible genes, T‐DNA insertion mutants of 10 B deficiency‐induced genes were obtained, and their growth properties in response to B conditions were examined. Among the lines examined, mutants of the transcription factor WRKY6 showed growth defect compared with the wild‐type under B deficiency, but not under normal conditions. This growth defect was commonly observed among three independently isolated wrky6 mutants. There was no significant difference in B concentration between wrky6‐3 and the wild‐type. Promoter activity of WRKY6 was induced around the root tip under B deficiency. These results established that WRKY6 is a low‐B‐induced transcription factor gene that is essential for normal root growth under low‐B conditions. Transcriptome analysis around the root tip identified WRKY6‐regulated genes under B deficiency. Our findings represent the first identification of a transcription factor involved in the response to B deficiency.