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Molecular characterization of putative vacuolar NHX‐type Na + /H + exchanger genes from the salt‐resistant tree Populus euphratica
Author(s) -
Ye ChuYu,
Zhang HeChen,
Chen JinHuan,
Xia XinLi,
Yin WeiLun
Publication year - 2009
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2009.01269.x
Subject(s) - populus euphratica , complementation , antiporter , sodium–hydrogen antiporter , vacuole , mutant , yeast , biology , gene , protein fragment complementation assay , botany , biochemistry , chemistry , cytoplasm , sodium , organic chemistry , membrane
The vacuolar NHX‐type Na + /H + exchangers play a key role in salt tolerance in plants. However, little is known about the Na + /H + exchangers in the salt‐resistant tree, Populus euphratica . In this study, we identified six putative vacuolar Na + /H + exchanger genes from P. euphratica , designated as PeNHX1–6 . Real‐time polymerase chain reaction indicated that the PeNHX1 /3/6 transcripts were abundant compared with the other three PeNHX genes in the three tissues (roots, stems and leaves) examined. After NaCl treatment for 6 h, the transcript levels of PeNHX1–6 were upregulated in the roots. To address the function of PeNHX1–6, complementation studies were performed with the salt‐sensitive yeast mutant strain R100, which lacks activity of the endosomal Na + /H + antiporter NHX1. The results showed that PeNHX1–6 compensates, at least in part, for the function of yeast NHX1. Moreover, PeNHX3 was targeted to the tonoplast when transiently expressed in onion. Together, these results suggest that PeNHX1–6 function as vacuolar Na + /H + exchangers and that PeNHX products play an important role in the salt resistance of P. euphratica .