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Methyl jasmonate dramatically enhances the accumulation of phenolic acids in Salvia miltiorrhiza hairy root cultures
Author(s) -
Xiao Ying,
Gao Shouhong,
Di Peng,
Chen Junfeng,
Chen Wansheng,
Zhang Lei
Publication year - 2009
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2009.01257.x
Subject(s) - rosmarinic acid , methyl jasmonate , salvia miltiorrhiza , cinnamic acid , biochemistry , caffeic acid , biosynthesis , phenylalanine ammonia lyase , chemistry , hairy root culture , biology , phenylalanine , enzyme , amino acid , gene , transformation (genetics) , medicine , alternative medicine , traditional chinese medicine , pathology , agrobacterium , antioxidant
The aim of this work was to examine rosmarinic acid and its derivative lithospermic acid B accumulation, as well as related gene transcript and metabolite profiling in Salvia miltiorrhiza Bunge (Lamiacae) hairy root cultures, in response to methyl jasmonate (0.1 m M ). Results showed methyl jasmonate dramatically enhanced both rosmarinic acid and lithospermic acid B accumulation, from approximately 3.25 to 6.02%, and 2.94 to 19.3% of dry weight, respectively. Meantime, several rosmarinic acid biosynthetic gene transcripts were coordinately induced, with phenylalanine ammonia‐lyase, cinnamic acid 4‐hydroxylase, tyrosine aminotransferase, 4‐hydroxyphenylpyruvate reductase and 4‐hydroxyphenylpyruvate dioxygenase transcripts displaying the most rapid and substantial increases. Liquid chromatographic–tandem mass spectrometry was used to characterize the profile of metabolites involved in rosmarinic acid biosynthesis pathway, in both control and elicited‐treated hairy root cultures. Further canonical correlation analysis constructed a gene‐to‐metabolite network, locating possible gene candidates which would directly link to phenolic acids (rosmarinic acid and lithospermic acid B) production, and thereby, would help to prompt the possibility of a key gene‐based metabolic engineering for the synthesis of active pharmaceutical compounds in S. miltiorrhiza .