Premium
Comparative genomic and physiological analysis of nutrient response to , : and in barley seedlings
Author(s) -
Lopes Marta S.,
Araus José L.
Publication year - 2008
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2008.01114.x
Subject(s) - hordeum vulgare , nitrate reductase , biology , biochemistry , nitrogen assimilation , nitrite reductase , gene expression , photosynthesis , cytochrome c oxidase , botany , gene , enzyme , poaceae
Long‐term differences in photosynthesis, respiration and growth of plants receiving distinct nitrogen (N) sources imply that N metabolism generates signals that regulate metabolism and development. The molecular basis of these signals remains unclear. Here we studied the gene expression profiles of barley ( Hordeum vulgare L. cv. Graphic) seedlings fertilized either with ammonium (), with ammonium and nitrate (:), or with nitrate () only. Our transcriptome analysis after 48 h of growth in these N sources showed major changes in the expression of genes involved in N metabolism (nitrate reductase), signalling (protein kinases and protein phosphatases), photosynthesis (chlorophyll a / b ‐binding protein and a PsbQ domain), where increases in as compared with were observed. Moreover, assimilation induced genes participating in C and sugars metabolism (phosphoglycerate kinase, glucosyltranferase and galactokinase), respiration (cytochrome c oxidase), protein fate (heat shock proteins) and development (MTN3‐like protein). These changes in gene expression could well explain the long‐term growth depression observed in plants. Even if a few genes participating in protein fate (proteases) and development (OsNAC5) were upregulated in as compared with :, the general pattern of expression was quite similar between these two N sources. Taken together, these results indicated that other downstream mechanisms should be involved in the synergetic long‐term response of :.