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How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore‐derived embryogenesis
Author(s) -
SeguíSimarro José M.,
Nuez Fernando
Publication year - 2008
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2008.01113.x
Subject(s) - microspore , embryogenesis , biology , maternal to zygotic transition , embryo , transcriptome , ploidy , microbiology and biotechnology , microspora , zygote , totipotent , doubled haploidy , somatic embryogenesis , botany , gene expression , genetics , gene , cellular differentiation , stamen , pollen
Microspore embryogenesis is the most powerful androgenic pathway to produce haploid and doubled haploid plants. To deviate a microspore toward embryogenesis, a number of factors, different for each species, must concur at the same time and place. Once induced, the microspore undergoes numerous changes at different levels, from overall morphology to gene expression. Induction of microspore embryogenesis not only implies the expression of an embryogenic program, but also a stress‐related cellular response and a repression of the gametophytic program to revert the microspore to a totipotent status. In this review, we compile the most recent advances in the understanding of the changes undergone by the induced microspore to readapt to the new developmental scenario. We devote special attention to the efforts made to uncover changes in the transcriptome of the induced microspore and microspore‐derived embryo (MDE). Finally, we discuss the influence that an in vitro environment exerts over the MDE, as compared with its zygotic counterpart.