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EIN3‐like gene expression during fruit ripening of Cavendish banana ( Musa acuminata cv. Grande naine)
Author(s) -
MbéguiéAMbéguié Didier,
Hubert Olivier,
FilsLycaon Bernard,
Chillet Marc,
Baurens FrancChristophe
Publication year - 2008
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2008.01083.x
Subject(s) - ripening , ethylene , musa acuminata , gene , biology , gene expression , transcription factor , botany , mads box , arabidopsis , horticulture , genetics , biochemistry , mutant , catalysis
Ethylene signal transduction initiates with ethylene binding at receptor proteins and terminates in a transcription cascade involving the EIN3/EIL transcription factors. Here, we have isolated four cDNAs homologs of the Arabidopsis EIN3/EIN3‐like gene, MA‐EILs ( Musa acuminata ethylene insensitive 3‐like) from banana fruit. Sequence comparison with other banana EIL gene already registered in the database led us to conclude that, at this day, at least five different genes namely MA‐EIL1 , MA‐EIL2/AB266318 , MA‐EIL3/AB266319 , MA‐EIL4/AB266320 and AB266321 exist in banana. Phylogenetic analyses included all banana EIL genes within a same cluster consisting of rice OsEILs , a monocotyledonous plant as banana. However, MA‐EIL1 , MA‐EIL2/AB266318 , MA‐EIL4/AB266320 and AB266321 on one side, and MA‐EIL3/AB266319 on the other side, belong to two distant subclusters. MA‐EIL mRNAs were detected in all examined banana tissues but at lower level in peel than in pulp. According to tissues, MA‐EIL genes were differentially regulated by ripening and ethylene in mature green fruit and wounding in old and young leaves. MA‐EIL2/AB266318 was the unique ripening‐ and ethylene‐induced gene; MA‐EIL1 , MA‐EIL4/Ab266320 and AB266321 genes were downregulated, while MA‐EIL3/AB266319 presented an unusual pattern of expression. Interestingly, a marked change was observed mainly in MA‐EIL1 and MA‐EIL3/Ab266319 mRNA accumulation concomitantly with changes in ethylene responsiveness of fruit. Upon wounding, the main effect was observed in MA‐EIL4/AB266320 and AB266321 mRNA levels, which presented a markedly increase in both young and old leaves, respectively. Data presented in this study suggest the importance of a transcriptionally step control in the regulation of EIL genes during banana fruit ripening.