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Patch clamp characterization of a non‐selective cation channel of ER membranes purified from Beta vulgaris taproots
Author(s) -
BritoArgáez Ligia,
CantoCanché Blondy,
Teresa HernándezSotomayor Soledad Maria,
MartínezEstevez Manuel,
Pottosin Igor I.
Publication year - 2008
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2007.01031.x
Subject(s) - conductance , biophysics , patch clamp , membrane , chemistry , pipette , permeability (electromagnetism) , chromatography , biochemistry , biology , receptor , mathematics , combinatorics
The ER fraction from red beet taproot was purified on sucrose gradient and giant liposomes, suitable for patch clamping, were formed by dehydration–rehydration of the lipid film. Single‐channel recordings on excised and attached patches revealed a large conductance (165 pS) cation (P Cl− /P K+ < 0.03) channel with equal conductance and relative permeability for Na + and K + . This non‐selective cation channel was also highly permeable for Ca 2+ . We failed to detect any single‐channel currents activated by a direct application of d ‐ myo ‐inositol 1,4,5 trisphosphate, despite the fact that the ER membranes were native.