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Role of phenylalanine ammonia‐lyase in heat pretreatment‐induced chilling tolerance in banana fruit
Author(s) -
Chen Jianye,
He Lihong,
Jiang Yueming,
Wang Yong,
Joyce Daryl C.,
Ji Zuoliang,
Lu Wangjin
Publication year - 2008
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2007.01013.x
Subject(s) - phenylalanine ammonia lyase , phenylalanine , chemistry , horticulture , ammonia , enzyme , enzyme assay , heat stress , food science , biochemistry , botany , biology , amino acid , zoology
Increasing evidence suggests that phenylalanine ammonia‐lyase (PAL, EC 4.3.1.5) is associated with low temperature stress in plant tissues. Banana fruit are highly susceptible to chilling injury. However, little is known about the role of PAL (i.e. gene expression, protein level and activity) in fruit chilling. In this work, the involvement of PAL induced by heat treatment (38°C for 3 days) prior to storage (8°C) in chilling tolerance was investigated. The PAL inhibitor 2‐aminoindan‐2‐phosphonic acid (AIP) was also used to further study the role of PAL in the chilling tolerance. The results showed that mRNA transcripts ( MaPAL1 and MaPAL2 ) and PAL protein levels increased during storage at chilling temperature. Heat treatment prior to storage alleviated chilling injury and enhanced PAL activity, protein amount and MaPAL1 and MaPAL2 transcript levels. The increases in parameters of PAL upon heat pretreatment were all inhibited by AIP treatment, which resulted in aggravation of chilling injury. Thus, these findings indicate that the induction of PAL by heat pretreatment was regulated at both the transcriptional and the translational levels and that PAL may play a role in heat pretreatment‐induced chilling tolerance of banana fruit.