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Modulation of SAMDC expression in Arabidopsis thaliana alters in vitro shoot organogenesis
Author(s) -
Hu WenWei,
Gong Haibiao,
Pua EngChong
Publication year - 2006
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2006.00799.x
Subject(s) - organogenesis , biology , arabidopsis , putrescine , polyamine , auxin , shoot , downregulation and upregulation , microbiology and biotechnology , spermidine , arabidopsis thaliana , biochemistry , botany , enzyme , mutant , gene
S ‐Adenosylmethionine decarboxylase (SAMDC, EC 4.1.4.50) is a rate‐limiting enzyme in the biosynthesis of polyamines (PAs) from putrescine. To gain more insight into the role of PAs in shoot organogenesis, a reverse genetic approach has been used to study in vitro shoot organogenesis by manipulating SAMDC expression in Arabidopsis . Up‐ and downregulation of SAMDC expression was achieved by transferring sense, antisense and double‐stranded Arabidopsis SAMDC complementary DNA constructs back into Arabidopsis via Agrobacterium tumefaciens . Results show that the biosynthesis of PAs and ethylene is mutually antagonistic by manipulation of SAMDC expression. Further results demonstrate that increased shoot organogenesis seems to be directly related to PA accumulation. This effect of PA may be further enhanced with reduced ethylene. It also suggests that spermidine is involved in the process of acquiring organogenesis competence through downregulation of ethylene production and shoot organogenesis, which might result from the concerted action of PAs and plant hormones such as auxin, cytokinins and ethylene.