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The mitochondrial type II peroxiredoxin from poplar
Author(s) -
Gama Filipe,
Keech Olivier,
Eymery Françoise,
Finkemeier Iris,
Gelhaye Eric,
Gardeström Per,
Dietz Karl Josef,
Rey Pascal,
Jacquot JeanPierre,
Rouhier Nicolas
Publication year - 2007
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2006.00785.x
Subject(s) - peroxiredoxin , reactive oxygen species , glutaredoxin , peroxidase , biology , biochemistry , mitochondrion , arabidopsis thaliana , thioredoxin , abiotic component , arabidopsis , microbiology and biotechnology , abiotic stress , botany , oxidative stress , enzyme , gene , mutant , paleontology
Mitochondria are a major site of reactive oxygen species production and controlling the peroxide levels in this compartment is essential. Peroxiredoxins (Prx) are heme‐free peroxidases, which use reactive cysteines for their catalysis and reducing systems for their regeneration. One of the two Prxs present in poplar mitochondria, Prx IIF, expressed as a recombinant protein, was found to reduce a broad range of peroxides with electrons provided preferentially by glutaredoxin and to a lesser extent by glutathione, all the thioredoxins tested being inefficient. This protein is constitutively expressed because it is found in all tissues analyzed. Its expression is modified during a biotic interaction between poplar and the rust fungus Melampsora laricii populina . On the other hand, Prx IIF expression does not substantially vary under abiotic stress conditions. Nevertheless, water deficit or chilling and probably induced senescence, but not photooxidative conditions or heavy metal treatment, also led to a small increase in PrxIIF abundance in Arabidopsis thaliana plants.