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Effects of sulfur limitation on photosystem II functioning in Chlamydomonas reinhardtii as probed by chlorophyll a fluorescence
Author(s) -
Antal Taras K.,
Volgusheva Alena A.,
Kukarskih Galina P.,
Bulychev Alexander A.,
Krendeleva Tatyana E.,
Rubin Andrey B.
Publication year - 2006
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2006.00734.x
Subject(s) - violaxanthin , chlamydomonas reinhardtii , photochemistry , photosystem ii , chlorophyll fluorescence , xanthophyll , chemistry , photosynthesis , chlorophyll , photosystem i , marchantia polymorpha , quenching (fluorescence) , fluorescence , zeaxanthin , lutein , carotenoid , biochemistry , physics , organic chemistry , quantum mechanics , gene , mutant
Chlorophyll fluorescence methods were applied to probe in vivo photosystem II (PSII) function in Chlamydomonas reinhardtii grown in sulfur‐depleted media under aerobic conditions. The rates of oxygen evolution and dark reduction decreased during a 24‐h incubation in sulfur‐deficient medium, while the respiration rate increased. The analysis of chlorophyll fluorescence induction curves suggests that electron transport was perturbed on both the acceptor and donor sides of PSII. Light‐induced violaxanthin de‐epoxidation and non‐photochemical fluorescence quenching were suppressed, owing to dark accumulation of zeaxanthin. Also sulfur‐deprived cells showed elevated concentrations of violaxanthin and lutein. Sulfur deprivation stimulated a pronounced (three‐ to four‐fold) increase in chlorophyll a fluorescence intensity (parameters F o and F m ), probably due to greater light absorption by carotenoids and changes in the excitation energy transfer and deactivation in PSII of C. reinhardtii .