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Expression patterns of low temperature responsive genes in a dominant ABA‐less‐sensitive mutant line of common wheat
Author(s) -
Kobayashi Fuminori,
Takumi Shigeo,
Egawa Chikako,
Ishibashi Machiko,
Nakamura Chiharu
Publication year - 2006
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2006.00689.x
Subject(s) - abscisic acid , mutant , biology , dormancy , gene , acclimatization , seedling , gene expression , microbiology and biotechnology , botany , genetics , germination
Abscisic acid (ABA) plays important role in mediating stress responses and in acquiring desiccation tolerance and dormancy of plant seeds. To study roles of ABA in cold acclimation and freezing tolerance in wheat, expression profiles of Cor/Lea and their putative transcription factor (TF) genes were analysed using a dominant mutation line of common wheat EH47‐1 lacking seed dormancy. The mutant line was less sensitive to exogenous ABA than the original line as judged by the magnitude of ABA inhibition of seedling growth. Expression analysis of Cor / Lea and TF genes however, showed that more transcripts were present in ABA‐treated seedlings of the mutant line. In developing caryopses, the same tendency was observed. The mutant line showed no changes in the cold acclimation ability, but it showed a higher level of freezing tolerance than the original line without cold acclimation. No significant differences were observed in the expression profiles of Cor / Lea and TF genes during cold acclimation between the two lines. Our results imply the presence of an unknown ABA‐dependent cold responsive pathway, which enhances the basal level of freezing tolerance by a dominant mutation in EH47‐1.