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Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions
Author(s) -
Cabrera Juan Carlos,
Messiaen Johan,
Cambier Pierre,
Van Cutsem Pierre
Publication year - 2006
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2006.00677.x
Subject(s) - acetylation , chemistry , chitosan , oligomer , phenylalanine ammonia lyase , programmed cell death , arabidopsis thaliana , degree of polymerization , desorption , cell wall , biochemistry , polymerization , polymer chemistry , phenylalanine , organic chemistry , apoptosis , amino acid , mutant , gene , polymer , adsorption
Chitosan oligomers are known elicitors of plant defence mechanisms. In this work, chitooligosaccharides of different degrees of polymerization and degrees of acetylation were prepared and characterized by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry. The effect of the degree of polymerization (DP), degree of acetylation and concentration of these chitooligosaccharides on defence activation in Arabidopsis thaliana suspension‐cultured cells was studied. Our study results show that fully deacetylated chitooligosaccharides (chitosan oligomers) induce, depending on their DP and concentration, phenylalanine ammonia‐lyase (PAL) activation, H 2 O 2 synthesis and cell death in A. thaliana cell suspensions. The progressive reacetylation of the chitosan oligomer elicitors progressively impaired their ability to enhance H 2 O 2 accumulation and cell death, but did not affect the activation of PAL.