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The function of SULTR2;1 sulfate transporter during seed development in Arabidopsis thaliana
Author(s) -
Awazuhara Motoko,
Fujiwara Toru,
Hayashi Hiroaki,
WatanabeTakahashi Akiko,
Takahashi Hideki,
Saito Kazuki
Publication year - 2005
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2005.00543.x
Subject(s) - silique , arabidopsis thaliana , arabidopsis , biology , cauliflower mosaic virus , transgene , genetically modified crops , wild type , reporter gene , microbiology and biotechnology , gene , mutant , botany , biochemistry , gene expression
SULTR2;1 is a low‐affinity sulfate transporter expressed in the vascular tissues of roots and leaves for interorgan transport of sulfate in Arabidopsis thaliana . Transgenic Arabidopsis carrying a fusion gene construct of SULTR2;1 5′‐promoter region and β‐glucuronidase coding sequence (GUS) demonstrated that within the reproductive tissues, SULTR2;1 is specifically expressed in the bases and veins of siliques and in the funiculus, which connects the seeds and the silique. The antisense suppression of SULTR2;1 mRNA caused decrease of sulfate contents in seeds and of thiol contents both in seeds and leaves, as compared with the wildtype (WT). The effect of antisense suppression of SULTR2;1 on seed sulfur status was determined by introducing a sulfur‐indicator construct, p35S::β SR x3:GUS, which drives the expression of GUS reporter under a chimeric cauliflower mosaic virus 35S promoter containing a triplicate repeat of sulfur‐responsive promoter region of soybean β‐conglycinin β subunit (β SR x3). The mature seeds of F1 plants carrying both the SULTR2;1 antisense and p35S::β SR x3:GUS constructs exhibited significant accumulation of GUS activities on sulfur deficiency, as compared with those carrying only the p35S::β SR x3:GUS construct in the WT background. These results suggested that SULTR2;1 is involved in controlling translocation of sulfate into developing siliques and may modulate the sulfur status of seeds in A. thaliana .