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Identification, accumulation, and functional prediction of novel tomato transcripts systemically upregulated after fire damage
Author(s) -
Coker Jeffrey Scott,
Vian Alain,
Davies Eric
Publication year - 2005
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2005.00488.x
Subject(s) - suppression subtractive hybridization , biology , lycopersicon , complementary dna , gene , downregulation and upregulation , microbiology and biotechnology , gene expression , symporter , cdna library , biochemistry , botany , transporter
Despite the major impacts of fire on plants, responses to fire damage have not been closely studied on the level of gene expression. Here, we present analyses of novel transcripts from tomato ( Lycopersicon esculentum cv. Heinz), which are systemically upregulated in leaves after a distant leaf is wounded by flame. Nine cDNA fragments were isolated from a subtractive cDNA library of leaf tissue 1 h after flaming. Using data mining and polymerase chain reaction (PCR), full‐length open‐reading frames were predicted, amplified, and then sequenced. Real‐time (RT)‐PCR using leaf RNA after flaming confirmed the systemic accumulation of 4 and 7 transcripts within 30 and 60 min, respectively, before returning to basal levels within 3 h. During this same time course, proteinase inhibitor I levels gradually increased over 30‐fold in 6 h. Expression analyses also showed that eight of the transcripts are present in unwounded leaf, stem, and root tissues. The predicted proteins include an acyl carrier, adenylyl sulfate reductase, PS II oxygen‐evolving complex protein 3, anion : sodium symporter, chloroplast‐specific ribosomal protein, a histidine triad family protein, and an unknown wound/stress‐related protein. Homologs of several of these proteins have been associated with other types of wound and stress responses. It appears that, within an hour after being damaged by fire, plants systemically upregulate a variety of genes involved with basic cell metabolism and upkeep, in addition to classic defense genes such as proteinase inhibitors.