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Immunolocalization of homogalacturonans in the apex of the long‐day plant Sinapis alba at floral transition. The pectin content drops dramatically in the first hours of this transition
Author(s) -
Sobry Stéphanie,
Havelange Andrée,
Liners Françoise,
Van Cutsem Pierre
Publication year - 2005
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2005.00463.x
Subject(s) - sinapis , pectin , meristem , apex (geometry) , cell wall , chemistry , botany , plant cell , transition (genetics) , biophysics , biology , biochemistry , brassica , shoot , gene
The pectin content in the meristem of Sinapis alba at flowering transition was assessed in electron microscopy using immunocytochemistry and the monoclonal antibody 2F4 that recognizes a Ca 2+ ‐induced supramolecular conformation of homogalacturonans (Liners et al. Plant Physiol 91: 1419–1424, 1989; Liners et al. Plant Physiol 99: 1099–1104, 1992). The meristem's pectins were not recognized at all by the 2F4 monoclonal antibody unless they were enzymatically (PME: pectin methylesterase) or chemically (NaOH) de‐esterified, indicating that native pectin must be largely esterified in the apical meristem. A striking but transient decrease of the homopolygalacturonic content of the meristem's cell walls occurred between 20 and 24 h after start of the inductive long day. These changes suggest a role for pectin in floral transition.