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Differentially expressed genes during seed development in soybean
Author(s) -
Beilinson Vadim,
Moskalenko Oleksandr V.,
Ritchie Rae D.,
Nielsen Niels C.
Publication year - 2005
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2005.00450.x
Subject(s) - biology , suppression subtractive hybridization , gene , complementary dna , cdna library , gene expression , reverse transcriptase , genetics , false positive paradox , polymerase chain reaction , microbiology and biotechnology , computational biology , machine learning , computer science
Little reliable information exists about the genetic events that control the onset and timing of seed‐fill in soybean cotyledons. To identify the genes involved in this process, cDNA libraries were prepared from mRNAs isolated from seeds at 7 and 21 days after flowering (DAF), which represent times just before and after the initiation of seed‐fill. For the soybean variety Resnik, which was used for this study, seed‐fill and the establishment of an endoreduplicative cell cycle occurred 12–14 DAF. Suppression subtractive hybridization was then applied to identify sequences that were differentially expressed at each of these two developmental stages. False positives in the libraries were reduced by using mirror orientation selection (MOS). The libraries of differentially expressed genes that resulted were analysed and the nucleotide sequences obtained were compared with those in existing databases. Several genes from each library were chosen and their expression profile during seed development was analysed by quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) using RNA preparations originating from different seed developmental stages. Candidate genes for control of the stage shift from dividing cells to endoreduplication were identified.