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Temporal and spatial expression of flavonoid biosynthetic genes in flowers of Anthurium andraeanum
Author(s) -
Collette Vern E.,
Jameson Paula E.,
Schwinn Kathy E.,
Umaharan Pathmanathan,
Davies Kevin M.
Publication year - 2004
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2004.00402.x
Subject(s) - anthurium , bract , chalcone synthase , araceae , biology , flavonoid biosynthesis , anthocyanin , botany , chalcone isomerase , genetics , gene , gene expression , inflorescence , transcriptome
The expression of anthocyanin biosynthesis genes during flower colour development in Anthurium andraeanum (anthurium) was studied. A cDNA library was constructed from mRNA from the anthurium spathe, and full‐length cDNA clones identified for the flavonoid biosynthetic enzymes chalcone synthase (CHS), flavanone 3‐hydroxylase (F3H), dihydroflavonol 4‐reductase (DFR) and anthocyanidin synthase (ANS). These were used to measure transcript levels in the spathe during flower development, in cultivars with different flower colours, over the diurnal cycle, and in the spadix. CHS , F3H and ANS were expressed at all stages of spathe and spadix development. However, DFR transcript levels varied significantly between stages, and DFR may represent a key point of regulation. A diurnal rhythm of DFR transcript abundance in the spathe was also observed, with transcript levels high at dawn and dusk and low at noon. Control of anthocyanin biosynthesis in anthurium spathe differs from that described for flowers of other species, with DFR a key regulatory point and a complex mix of developmental and environmental control signals.