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Changes in chlorophyll and carotenoid contents in radish ( Raphanus sativus ) cotyledons show different time courses during senescence
Author(s) -
Suzuki Yasuyo,
Shioi Yuzo
Publication year - 2004
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2004.00401.x
Subject(s) - raphanus , carotenoid , chlorophyll , senescence , lutein , violaxanthin , biology , botany , carotene , chlorophyll a , high performance liquid chromatography , zeaxanthin , chemistry , chromatography , microbiology and biotechnology
Changes of chlorophylls and carotenoids from green to yellow cotyledons of the radish ( Raphanus sativus ) were simultaneously and systematically analysed by high‐performance liquid chromatography (HPLC) with photodiode array detection. Twenty‐one components, seven chlorophylls and 14 carotenoids, were detectable. Seven chlorophylls and five carotenoids were identified from the results of HPLC analyses. Most chlorophyll species degraded during senescence, whereas carotenoids showed different behaviour in their metabolism depending on pigment species. For instance, during senescence, the contents of lutein and violaxanthin changed only slightly, β‐carotene in 5‐day‐senescent cotyledons became 2.7 times higher than non‐senescent leaves. Carotenoids of radish cotyledons were classified into three groups by their changes in concentration during senescence (increased, degraded and constant) and their roles discussed.