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Different arabinogalactan proteins are present in carrot ( Daucus carota ) cell culture medium and in seeds
Author(s) -
Immerzeel Peter,
Schols Henk A.,
Voragen Alphons G. J.,
De Vries Sacco C.
Publication year - 2004
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2004.00395.x
Subject(s) - daucus carota , arabinose , arabinogalactan , glucuronic acid , galactose , biochemistry , rhamnose , chemistry , polysaccharide , cell wall , glycan , xylose , biology , botany , fermentation , glycoprotein
Arabinogalactan proteins (AGPs) were isolated by Yariv phenylglycoside precipitation from the medium of carrot ( Daucus carota L.) cell cultures and from carrot seeds. The isolates showed a different composition of AGPs. The medium AGPs contained an arabinose poor AGP fraction that had relatively high levels of glucuronic acid and rhamnose. In contrast the seed AGPs only contained arabinose and galactose‐rich AGP fractions that had low levels of glucuronic acid. Linkage analysis on all fractions showed that most of the arabinose residues were terminally linked and that almost all galactose was present in the 1,3‐, 1,6‐ and 1,3,6‐ form. The strongly branched type II arabinogalactans are characteristic of the carbohydrate part of AGPs. AGP characteristic amino acid residues as Hyp, Pro, Glx, Ser, Gly, Asx, Ala, Leu and Thr were detected in three different fractions.