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cDNA cloning and characterization of a new stress‐responsive gene BoRS1 from Brassica oleracea var. acephala
Author(s) -
Tang Dongqin,
Qian Hongmei,
Yu Shunwu,
Cao Youfang,
Liao Zhihua,
Zhao Lingxia,
Sun Xiaofen,
Huang Danfeng,
Tang Kexuan
Publication year - 2004
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.2004.00369.x
Subject(s) - brassica oleracea , complementary dna , biology , southern blot , open reading frame , gene , rapid amplification of cdna ends , abscisic acid , northern blot , arabidopsis thaliana , genbank , genomic dna , microbiology and biotechnology , genetics , botany , molecular cloning , mutant , peptide sequence
A new stress‐responsive gene BoRS1 (GenBank accession No. AY373021 ) was isolated from Brassica oleracea var. acephala by rapid amplification of cDNA ends (RACE). The full‐length cDNA of BoRS1 was 2076 bp and contained a 1851 bp open reading frame (ORF) encoding 617 amino acids. Sequence analysis indicated that the deduced BoRS1 shared some identities with LTI65 , RD29A, RD29B and COR78 from Arabidopsis thaliana . Southern blot analysis of genomic DNA indicated that other related genes existed and there were two copies of BoRS1 in the genome of B. oleracea . Northern blot analysis revealed that BoRS1 was up‐regulated by cold, mannitol, NaCl and abscisic acid (ABA). Expressional fluctuation of time course with ABA implied a two‐step induction process. Tissue‐specific expression analysis indicated that BoRS1 was expressed in all the tested plant tissues including leaves, stems and roots. Our studies imply that BoRS1 is a new gene that is responsive to environmental stresses such as low temperature, salinity and osmotic stress.