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Stress‐induced formation of haploid plants through anther culture in cork oak ( Quercus suber )
Author(s) -
Bueno Maria Angeles,
Gómez Arancha,
Boscaiu Monica,
Manzanera José Antonio,
Vicente Oscar
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb05421.x
Subject(s) - microspore , quercus suber , stamen , botany , biology , doubled haploidy , ploidy , sucrose , horticulture , cork , biochemistry , gene , pollen
Induction of haploid embryos and regeneration of plantlets have been obtained, for the first time, in cork oak ( Quercus suber L.) by combining a starvation treatment in anther culture with a mild heat shock at 33°C for 5 days, followed by culture at 25°C in a simple agar medium without growth regulators. The same conditions had been shown previously to be optimal for embryogenic induction in isolated microspore cultures of several model species such as tobacco and wheat. These results support the notion that stress, particularly sucrose starvation, a heat shock or a combination of both treatments could be the major and general signal responsible for the inhibition of normal gametophytic development of the microspores and for the induction of the alternative embryogenic pathway. A similar approach may be used for the production of haploid and doubled haploids for plant breeding in other species that, like most forest trees, are still recalcitrant in anther culture.