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nif V is contiguous to nif HDK in Frankia strain FaC1
Author(s) -
Oh Bo Si,
Twigg Paul G.,
Hong Jeong Soo,
Mullin Beth C.,
An Chung Sun
Publication year - 1997
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1997.tb05375.x
Subject(s) - cosmid , orfs , biology , frankia , genetics , gene , open reading frame , subcloning , microbiology and biotechnology , sequence analysis , nucleic acid sequence , strain (injury) , restriction map , plasmid , nitrogen fixation , peptide sequence , root nodule , anatomy , bacteria
The organization of genes with the capacity to code for four proteins involved in nitrogen fixation in Frankia strain FaC1 was determined by restriction fragment mapping and nucleotide sequence analysis. Analysis of the 44‐kb genomic cosmid clone pFAH 1. isolated from a cosmid library made from Frankia strain FaCl, resulted in the identification of a 7.2‐kb Pst I fragment to which Klebsiella nif H, nif D and nif K probes hybridized. This nif ‐hybridizing fragment was subcloned and analyzed by restriction fragment mapping. Further subcloning of the 7.2‐kb fragment and subsequent sequence analysis of approximately 6.8 kb revealed the presence of six open reading frames (ORFs). Four of these ORFs have the potential to code for nif V‐, nif H‐, nif D‐ and nif K‐like gene products and the two others are unidentified ORFs. The organization of the structural genes for nitrogenase is the same in this Frankia strain as it is in most other nitrogen‐fixing prokaryotes, but the positioning of the nif V‐like gene relative to the nif HDK cluster differs, A consensus nif ‐promoter‐like sequence, found 5’to nif H. was not detected upstream of the nif V‐like gene. Nine copies of a 7‐bp direct repeat were found 5’to ORFA.